Production of Functional Glucagon-Secreting α-Cells From Human Embryonic Stem Cells

Production of Functional Glucagon-Secreting α-Cells From Human Embryonic Stem Cells

OBJECTIVE Differentiation of human embryonic stem (hES) cells to fully developed cell types holds great therapeutic promise. Despite significant progress, the conversion of hES cells to stable, fully differentiated endocrine cells that exhibit physiologically regulated hormone secretion has not yet been achieved. Here we describe an efficient differentiation protocol for the in vitro conversion...

Production of Functional Glucagon-Secreting Alpha Cells from Human Embryonic Stem Cells

Alireza Rezania, Michael J. Riedel, Rhonda D. Wideman, Francis Karanu, Ziliang Ao, Garth L. Warnock, and Timothy J. Kieffer BetaLogics Venture, Centocor Research & Development, Skillman, NJ, USA; Laboratory of Molecular and Cellular Medicine, Department of Cellular & Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, BC, Canada, V6T 1Z3; Department of Su...

differentiation of human embryonic stem cells into insulin- secreting cells

introduction: type i diabetes mellitus is caused by autoimmune destruction of the insulin-producing β-cells. a new potential method for curing the disease is transplantation of differentiated insulin- secreting cells from human embryonic stem cells. methods: human embryonic stem cell lines (royan h1) were used to produce embryoid bodies. differentiation carried out by growth factor-mediated sel...

Differentiation of human embryonic stem cells into neurons

Human embryonic stem (ES) cells are undifferentiated pluripotent cells derived from the inner cell mass of blastocyst stage embryos. These unique cell lines have the potential to form virtually any cell type in the body and can be propagated in vitro indefinitely in an undifferentiated state. These cells are capable of forming embryoid bodies (EB) that contain cells from all three embryonic lin...

Functional Concentrations of BMP4 on Differentiation of Mouse Embryonic Stem Cells to Primordial Germ Cells